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Science. 2000 Mar 24;287(5461):2271-4.

A BAC-based physical map of the major autosomes of Drosophila melanogaster.

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  • 1Berkeley Drosophila Genome Project, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. hoskins@bdgp.lbl.gov

Erratum in

  • Science 2000 Jun 9;288(5472):1751.

Abstract

We constructed a bacterial artificial chromosome (BAC)-based physical map of chromosomes 2 and 3 of Drosophila melanogaster, which constitute 81% of the genome. Sequence tagged site (STS) content, restriction fingerprinting, and polytene chromosome in situ hybridization approaches were integrated to produce a map spanning the euchromatin. Three of five remaining gaps are in repeat-rich regions near the centromeres. A tiling path of clones spanning this map and STS maps of chromosomes X and 4 was sequenced to low coverage; the maps and tiling path sequence were used to support and verify the whole-genome sequence assembly, and tiling path BACs were used as templates in sequence finishing.

PMID:
10731150
[PubMed - indexed for MEDLINE]
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