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Int J Food Microbiol. 1999 Feb 2;46(2):105-12.

Screening of non-Saccharomyces wine yeasts for the production of beta-D-xylosidase activity.

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  • 1Departamento de Biotecnologia de Alimentos, Instituto de Agroquímica y Tecnologia de Alimentos (CSIC), Valencia, Spain.


Fifty-four yeast strains belonging to the genera Candida, Dekkera, Hanseniaspora, Metschnikowia, Pichia, Rhodotorula, Schizosaccharomyces and Zygosaccharomyces, mainly isolated from grapes and wines, were screened for the production of beta-D-xylosidase activity. Beta-D-xylosidase activity was only detected in eight yeast strains belonging to the genera Hanseniaspora (H. osmophila and H. uvarum) and Pichia (P. anomala). Beta-D-xylosidase preparations active against p-nitrophenyl-beta-D-xyloside were characterised with respect to their optimal pH and temperature conditions. H. uvarum 11105 and 11107 and P. anomala 10320 beta-D-xylosidase preparations were active at pH and temperature ranges and at concentrations of glucose and ethanol usually found during winemaking processes.

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