Yra1p, a conserved nuclear RNA-binding protein, interacts directly with Mex67p and is required for mRNA export

EMBO J. 2000 Feb 1;19(3):410-20. doi: 10.1093/emboj/19.3.410.

Abstract

Mex67p and Mtr2p constitute an essential mRNA export complex that interacts with poly(A)+ RNA and nuclear pore proteins. We have identified Yra1p, an intranuclear protein with in vitro RNA-RNA annealing activity, which directly binds to Mex67p. The complex between Yra1p and Mex67p was reconstituted in vitro and shown by UV-crosslinking to bind directly to RNA. Mutants of YRA1 are impaired in nuclear poly(A)+ RNA export at restrictive growth conditions. ALY, the mouse homologue of Yra1p and a transcriptional coactivator, can bind in vitro to yeast and human Mex67p and partly complements the otherwise non-viable yra1 null mutant. Thus, Yra1p is the first RNA-binding protein characterized, which bridges the shuttling Mex67p/Mtr2p exporter to intranuclear mRNA transport cargoes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Biological Transport
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism*
  • Humans
  • Mice
  • Microscopy, Fluorescence
  • Molecular Sequence Data
  • Mutation
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism*
  • Nucleocytoplasmic Transport Proteins*
  • Poly A / genetics
  • Protein Binding
  • RNA, Messenger / metabolism*
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / metabolism*
  • Saccharomyces cerevisiae Proteins*
  • Sequence Homology, Amino Acid
  • Transcription Factors / metabolism
  • Yeasts / genetics

Substances

  • ALYREF protein, human
  • Fungal Proteins
  • MEX67 protein, S cerevisiae
  • Nuclear Proteins
  • Nucleocytoplasmic Transport Proteins
  • RNA, Messenger
  • RNA-Binding Proteins
  • Refbp1 protein, mouse
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • YRA1 protein, S cerevisiae
  • Poly A