Abstract
Phospholipase C and two inositol polyphosphate (IP) kinases constitute a signaling pathway that regulates nuclear messenger RNA export through production of inositol hexakisphosphate (IP6). The inositol 1,4,5-trisphosphate kinase of this pathway in Saccharomyces cerevisiae, designated Ipk2, was found to be identical to Arg82, a regulator of the transcriptional complex ArgR-Mcm1. Synthesis of inositol 1,4,5,6-tetrakisphosphate, but not IP6, was required for gene regulation through ArgR-Mcm1. Thus, the phospholipase C pathway produces multiple IP messengers that modulate distinct nuclear processes. The results reveal a direct mechanism by which activation of IP signaling may control gene expression.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Arginine / metabolism
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Cell Nucleus / enzymology
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Cell Nucleus / metabolism*
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DNA-Binding Proteins / metabolism
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Gene Expression Regulation, Fungal*
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Inositol 1,4,5-Trisphosphate / metabolism
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Inositol Phosphates / metabolism*
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Minichromosome Maintenance 1 Protein
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Molecular Sequence Data
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Phosphoric Monoester Hydrolases / metabolism
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Phosphorylation
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Phosphotransferases (Alcohol Group Acceptor) / chemistry
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Phosphotransferases (Alcohol Group Acceptor) / metabolism*
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Phytic Acid / metabolism
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Saccharomyces cerevisiae / enzymology
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Saccharomyces cerevisiae / genetics*
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Signal Transduction
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Transcription Factors / metabolism
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Transcription, Genetic*
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Type C Phospholipases / metabolism
Substances
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DNA-Binding Proteins
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Inositol Phosphates
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Minichromosome Maintenance 1 Protein
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Transcription Factors
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inositol-1,4,5,6-tetrakisphosphate
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Phytic Acid
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Inositol 1,4,5-Trisphosphate
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Arginine
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Phosphotransferases (Alcohol Group Acceptor)
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Inositol 1,4,5-trisphosphate 3-kinase
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inositol 1,3,4,5,6-pentakisphosphate-1-3-phosphatase
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Phosphoric Monoester Hydrolases
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Type C Phospholipases