Abstract
A gene cluster upstream of the arylsulfatase gene (atsA) in Pseudomonas aeruginosa was characterized and found to encode a putative ABC-type transporter, AtsRBC. Mutants with insertions in the atsR or atsB gene were unable to grow with hexyl-, octyl-, or nitrocatecholsulfate, although they grew normally with other sulfur sources, such as sulfate, methionine, and aliphatic sulfonates. AtsRBC therefore constitutes a general sulfate ester transport system, and desulfurization of aromatic and medium-chain-length aliphatic sulfate esters occurs in the cytoplasm. Expression of the atsR and atsBCA genes was repressed during growth with sulfate, cysteine, or thiocyanate. No expression of these genes was observed in the cysB mutant PAO-CB, and the ats genes therefore constitute an extension of the cys regulon in this species.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Motifs
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Amino Acid Sequence
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Arylsulfatases / genetics*
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Bacterial Proteins / genetics
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Bacterial Proteins / physiology*
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Biological Transport / genetics
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Cloning, Molecular
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Esters / metabolism
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Gene Expression Regulation, Bacterial / drug effects
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Genes, Bacterial / genetics
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Genes, Bacterial / physiology
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Molecular Sequence Data
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Multigene Family
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Mutation / genetics
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Pseudomonas aeruginosa / enzymology
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Pseudomonas aeruginosa / genetics*
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Pseudomonas aeruginosa / growth & development
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Pseudomonas aeruginosa / metabolism
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Regulon / genetics*
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Sulfates / metabolism
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Sulfur / metabolism*
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Sulfur / pharmacology
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Trans-Activators / genetics
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Trans-Activators / physiology
Substances
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Bacterial Proteins
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CysB protein, Bacteria
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Esters
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Sulfates
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Trans-Activators
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Sulfur
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Arylsulfatases