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Biol Pharm Bull. 2000 Feb;23(2):204-7.

Evaluation of L-glutamate clearance capacity of cultured rat cortical astrocytes.

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  • 1Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, The University of Tokyo, Japan.

Abstract

Astrocytes have a function in the uptake of excitatory neurotransmitter L-glutamate via the glutamate transporter. To evaluate the L-glutamate clearance capacity of astrocytes, we developed a colorimetric method for the determination of L-glutamate concentration and measured changes in extracellular L-glutamate concentration in rat cortical astrocyte cultures. When L-glutamate (50-200 microM) was added to astrocyte cultures and incubated for 1-8 h, the extracellular L-glutamate concentration declined with time. When L-glutamate was mixed with astrocyte culture supernatants only, no significant change in L-glutamate concentration was observed, ruling out the possibility that L-glutamate is spontaneously or enzymatically degraded in the extracellular space. Alternatively, the time-dependent decline of extracellular L-glutamate concentration was blocked by the presence of glutamate uptake inhibitors, indicating that the glutamate uptake system of astrocytes plays a major role in the clearance of extracellular L-glutamate.

PMID:
10706385
[PubMed - indexed for MEDLINE]
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