Trends Biochem Sci. 2000 Mar;25(3):99-104.

Mapping chromosomal proteins in vivo by formaldehyde-crosslinked-chromatin immunoprecipitation.

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DIBIT HSR Biomedical Scientific Park, Via Olgettina 58, 20132 Milano, Italy. v.orlando@hsr.it

Abstract

Gene regulation is a complex process. Numerous factors appear to be required for the accurate temporal and spatial regulation of each gene. Often these factors are assembled into multiprotein complexes, contributing to specific gene regulation events. Understanding how all these factors are organized in the chromosome and how their function is regulated in vivo is a challenging task. One of the most useful techniques for studying this level of gene regulation is the in vivo fixation by formaldehyde crosslinking of proteins to proteins and proteins to DNA, followed by immunoprecipitation of the fixed material.

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Mapping chromosomal proteins in vivo by formaldehyde-crosslinked-chromatin immun...
Mapping chromosomal proteins in vivo by formaldehyde-crosslinked-chromatin immunoprecipitation.
Trends Biochem Sci. 2000 Mar ;25(3):99-104.

PubMed
Characterizing transcription factor binding sites using formaldehyde crosslinkin...
Characterizing transcription factor binding sites using formaldehyde crosslinking and immunoprecipitation.
Methods. 2002 Jan ;26(1):48-56.

PubMed
In vivo cross-linking and immunoprecipitation for studying dynamic Protein:DNA a...
In vivo cross-linking and immunoprecipitation for studying dynamic Protein:DNA associations in a chromatin environment.
Methods. 1999 Nov ;19(3):425-33.

PubMed
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Identification of unknown target genes of human transcription factors using chromatin immunoprecipitation.
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Mapping chromosomal proteins in vivo by formaldehyde-crosslinked-chromatin immunoprecipitation.

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