Proc Natl Acad Sci U S A. 1976 Nov;73(11):4070-4.

Tubulin requires tau for growth onto microtubule initiating sites.

Witman GB, Cleveland DW, Weingarten MD, Kirschner MW.

Abstract

Tubulin purified by phosphocellulose chromatography and free of accessory proteins will not form microtubules in the absence or presence of microtubule initiating sites (flagellar microtubules). The capacity for growth onto pre-existing "seeds" can be restored by the addition of small quantities of partially purified tau protein. Larger quantities restore the capacity for spontaneous assembly. These results suggest that tubulin requires tau for both initiation and growth of microtubules and that tau is incorporated into the microtubule throughout its length.

PMID:: 1069293; [PubMed - indexed for MEDLINE]
PMCID:: PMC431332

Free PMC Article

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, U.S. Gov't, P.H.S.

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

Labome Researcher Resource - ExactAntigen/Labome

Supplemental Content

Save items

Related citations in PubMed

In vitro assembly of pure tubulin into microtubules in the absence of microtubule-associated proteins and glycerol. [Proc Natl Acad Sci U S A. 1977]
In vitro assembly of pure tubulin into microtubules in the absence of microtubule-associated proteins and glycerol.
Herzog W, Weber K. Proc Natl Acad Sci U S A. 1977 May; 74(5):1860-4.
Effect of estramustine phosphate on the assembly of trypsin-treated microtubules and microtubules reconstituted from purified tubulin with either tau, MAP2, or the tubulin-binding fragment of MAP2. [Arch Biochem Biophys. 1987]
Effect of estramustine phosphate on the assembly of trypsin-treated microtubules and microtubules reconstituted from purified tubulin with either tau, MAP2, or the tubulin-binding fragment of MAP2.
Fridén B, Wallin M, Deinum J, Prasad V, Luduena R. Arch Biochem Biophys. 1987 Aug 15; 257(1):123-30.
Tubulin assembly protein: immunochemical and immunofluorescent studies on its function and distribution in microtubules and cultured cells. [Cell. 1978]
Tubulin assembly protein: immunochemical and immunofluorescent studies on its function and distribution in microtubules and cultured cells.
Lockwood AH. Cell. 1978 Apr; 13(4):613-27.
Review Biochemistry and physiology of microtubules. [Annu Rev Biochem. 1976]
Review Biochemistry and physiology of microtubules.
Snyder JA, McIntosh JR. Annu Rev Biochem. 1976; 45:699-720.
Review The control of microtubule assembly in vivo. [Int Rev Cytol. 1979]
Review The control of microtubule assembly in vivo.
Raff EC. Int Rev Cytol. 1979; 59:1-96.

See reviews... See all...

Cited by 14 PubMed Central articles

Review The many faces of tau. [Neuron. 2011]
Review The many faces of tau.
Morris M, Maeda S, Vossel K, Mucke L. Neuron. 2011 May 12; 70(3):410-26.
Seeding of normal Tau by pathological Tau conformers drives pathogenesis of Alzheimer-like tangles. [J Biol Chem. 2011]
Seeding of normal Tau by pathological Tau conformers drives pathogenesis of Alzheimer-like tangles.
Guo JL, Lee VM. J Biol Chem. 2011 Apr 29; 286(17):15317-31. Epub 2011 Mar 3.
Human microtubule-associated-protein tau regulates the number of protofilaments in microtubules: a synchrotron x-ray scattering study. [Biophys J. 2009]
Human microtubule-associated-protein tau regulates the number of protofilaments in microtubules: a synchrotron x-ray scattering study.
Choi MC, Raviv U, Miller HP, Gaylord MR, Kiris E, Ventimiglia D, Needleman DJ, Kim MW, Wilson L, Feinstein SC, et al. Biophys J. 2009 Jul 22; 97(2):519-27.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Tubulin requires tau for growth onto microtubule initiating sites.
Tubulin requires tau for growth onto microtubule initiating sites.
Proc Natl Acad Sci U S A. 1976 Nov ;73(11):4070-4.

PubMed
Bowman Lecture 1998. Diabetic retinopathy: some cellular, molecular and therapeu...
Bowman Lecture 1998. Diabetic retinopathy: some cellular, molecular and therapeutic considerations.
Eye (Lond). 1999 Aug ;13 ( Pt 4):497-523.

PubMed
Placebos and placebo effects in medicine: historical overview.
Placebos and placebo effects in medicine: historical overview.
J R Soc Med. 1999 Oct ;92(10):511-5.

PubMed
Regulation of ovarian extracellular matrix remodelling by metalloproteinases and...
Regulation of ovarian extracellular matrix remodelling by metalloproteinases and their tissue inhibitors: effects on follicular development, ovulation and luteal function.
J Reprod Fertil Suppl. 1999 ;54:367-81.

PubMed
Role of proteolytic enzymes in biological regulation (a review).
Role of proteolytic enzymes in biological regulation (a review).
Proc Natl Acad Sci U S A. 1976 Nov ;73(11):3825-32.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: