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Biochem Biophys Res Commun. 2000 Feb 24;268(3):677-82.

Cloning and expression of cytochrome P450 genes belonging to the CYP4 family and to a novel family, CYP48, in two hymenopteran insects, Trichogramma cacoeciae and Apis mellifera.

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  • 1INRA-IFR 38 "Réponse des organismes aux stress physico-chimiques et biologiques,", Unité Santé Végétale et Environnement, 123 boulevard Francis Meilland, Antibes Cedex, 06606, France. tares@antibes.inra.fr

Abstract

Cytochrome P450 partial sequences were isolated by PCR using genomic DNA from two hymenopteran insects of agronomical importance, Trichogramma cacoeciae, a parasitoid wasp, and Apis mellifera, the honeybee. Four new P450 genes were identified: one honeybee gene belongs to the CYP4 family and was named CYP4G11; the three other genes were from Trichogramma and belong to the CYP4 family (CYP4G12) and to a novel family, the CYP48 one (CYP48A1 and CYP48A2). The four genes contain a short intron (72-95 bp) at the same position as already described for other insect species. The two genes CYP48A1 and CYP48A2 have a supernumary intron (57-71 bp) upstream the first one. Only the two CYP4 genes were constitutively transcribed, at a high level for CYP4G12 and at a low level for CYP4G11. No expression was observed for CYP48A1 and CYP48A2.

Copyright 2000 Academic Press.

PMID:
10679264
[PubMed - indexed for MEDLINE]
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