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J Bacteriol. 2000 Mar;182(5):1437-41.

Mutational analysis of ligand recognition by tcp, the citrate chemoreceptor of Salmonella enterica serovar typhimurium.

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  • 1Department of Biotechnology, Division of Utilization of Biological Resources, Faculty of Agriculture, Gifu University, Gifu 501-1193, Japan.

Abstract

The chemoreceptor Tcp mediates taxis to citrate. To identify citrate-binding residues, we substituted cysteine for seven basic or polar residues that are chosen based on the comparison of Tcp with the well-characterized chemoreceptors. The results suggest that Arg-63, Arg-68, Arg-72, Lys-75, and Tyr-150 (and probably other unidentified residues) are involved in the recognition of citrate.

PMID:
10671471
[PubMed - indexed for MEDLINE]
PMCID:
PMC94436
Free PMC Article
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