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J Histochem Cytochem. 2000 Jan;48(1):89-94.

Brush border myosin I (BBMI): a basally localized transcript in human jejunal enterocytes.

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  • 1Division of Pediatric Gastroenterology/Nutrition, The Floating Hospital for Children, New England Medical Center Hospitals, Boston, Massachusetts 02111, USA.

Abstract

To extend our recent observation that villin mRNA, encoding an apical microvillous protein, is dichotomously localized in the basal region of human enterocytes, we examined the localization of mRNAs for brush border myosin I (BBMI) and intestinal fimbrin (I-fim). In situ hybridization indicated that BBMI mRNA localized to the basal region of human enterocytes, whereas the mRNA for I-fim distributed diffusely. To facilitate study of potential mechanisms of mRNA targeting, we cloned a full-length cDNA for BBMI including its 5'- and 3'-untranslated regions (UTRs). This cDNA shares 86% sequence identity with bovine BBMI and 85% with rat BBMI. Sequence analysis revealed no obvious similarity between the 3'-UTRs of BBMI and villin. This study provides evidence of novel sorting pathways for intestinal microvillous cytoskeletal proteins.

PMID:
10653589
[PubMed - indexed for MEDLINE]
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