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Pflugers Arch. 2000;439(3 Suppl):R152-3.

Ca2+-sensitive and Ca2+-insensitive exocytosis in maize coleoptile protoplasts.

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  • 1A.v.H. Inst. Plant Sciences, Göttingen.

Abstract

Ca2+ and osmotic driven extension of the surface area of maize coleoptile protoplasts was investigated using capacitance measurements and photolysis of the caged compound DM-nitrophen. Protoplasts responded to an elevation of cytoplasmic Ca2+ (Ca(i)) with a rapid burst in capacitance reaching a maximal increase of 1.3+/-1.1% over the resting cell capacitance. Subsequent lowering of the osmotic potential in the external medium by 210 mosmol caused a further increase in Cm by 26+/-6%. These data indicate two independent pathways for insertion of membrane into the plasma membrane. One is driven by Ca(i) and recruits membrane from a small pool. The osmotic evoked rise in surface area draws membrane from a much larger reservoir and may be driven by membrane tension.

PMID:
10653175
[PubMed - indexed for MEDLINE]
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