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J Virol Methods. 2000 Jan;84(1):95-8.

Inhibitors of RT-PCR in serum.

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  • 1Plum Island Animal Disease Center, Greenport, NY 11944, USA.


Amplification by RT-PCR of the RNA present in foot-and-mouth disease virus particles is inhibited by substances present in the sera of several species. This inhibition appears to be caused by a direct interaction of the substances with the RNA and not the enzymes used for its amplification.

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