Signalling via ADP-ribosylation factor 6 lies downstream of phosphatidylinositide 3-kinase

Biochem J. 2000 Feb 1;345 Pt 3(Pt 3):719-24.

Abstract

ADP-ribosylation factor (ARF) 6 regulates plasma membrane trafficking and cortical actin formation by cycling between inactive GDP and active GTP-bound conformations. Here we show that agonist stimulation of phosphatidylinositide 3-kinase (PI 3-kinase) activates a pathway that leads to ARF6 activation. We also describe experiments that propose a central role in this pathway for the PI 3-kinase-dependent plasma membrane recruitment of the cytohesin-1 family of PtdIns(3,4,5)P(3)-binding ARF-exchange factors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ADP-Ribosylation Factor 6
  • ADP-Ribosylation Factors / drug effects
  • ADP-Ribosylation Factors / genetics
  • ADP-Ribosylation Factors / metabolism*
  • Actins / metabolism
  • Actins / ultrastructure
  • Amino Acid Motifs
  • Amino Acid Sequence
  • Androstadienes / pharmacology
  • Brefeldin A / pharmacology
  • Cell Adhesion Molecules / drug effects
  • Cell Adhesion Molecules / metabolism
  • Cell Membrane / metabolism
  • Chromones / pharmacology
  • Culture Media, Serum-Free
  • Enzyme Inhibitors / pharmacology
  • Epidermal Growth Factor / pharmacology
  • Green Fluorescent Proteins
  • Guanine Nucleotide Exchange Factors
  • Guanosine Diphosphate / metabolism
  • HeLa Cells / drug effects
  • HeLa Cells / metabolism
  • Humans
  • Luminescent Proteins / genetics
  • Luminescent Proteins / metabolism
  • Molecular Sequence Data
  • Morpholines / pharmacology
  • Mutation
  • Phosphatidylinositol 3-Kinases / drug effects
  • Phosphatidylinositol 3-Kinases / metabolism*
  • Phosphatidylinositol Phosphates / antagonists & inhibitors
  • Phosphatidylinositol Phosphates / genetics
  • Phosphatidylinositol Phosphates / metabolism
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Signal Transduction
  • Subcellular Fractions
  • Wortmannin

Substances

  • ADP-Ribosylation Factor 6
  • Actins
  • Androstadienes
  • Cell Adhesion Molecules
  • Chromones
  • Culture Media, Serum-Free
  • Enzyme Inhibitors
  • Guanine Nucleotide Exchange Factors
  • Luminescent Proteins
  • Morpholines
  • Phosphatidylinositol Phosphates
  • Recombinant Proteins
  • cytohesin-1
  • phosphatidylinositol 3,4,5-triphosphate
  • Guanosine Diphosphate
  • Green Fluorescent Proteins
  • Brefeldin A
  • 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
  • Epidermal Growth Factor
  • ADP-Ribosylation Factors
  • ARF6 protein, human
  • Wortmannin