Abstract
ADP-ribosylation factor (ARF) 6 regulates plasma membrane trafficking and cortical actin formation by cycling between inactive GDP and active GTP-bound conformations. Here we show that agonist stimulation of phosphatidylinositide 3-kinase (PI 3-kinase) activates a pathway that leads to ARF6 activation. We also describe experiments that propose a central role in this pathway for the PI 3-kinase-dependent plasma membrane recruitment of the cytohesin-1 family of PtdIns(3,4,5)P(3)-binding ARF-exchange factors.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ADP-Ribosylation Factor 6
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ADP-Ribosylation Factors / drug effects
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ADP-Ribosylation Factors / genetics
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ADP-Ribosylation Factors / metabolism*
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Actins / metabolism
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Actins / ultrastructure
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Amino Acid Motifs
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Amino Acid Sequence
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Androstadienes / pharmacology
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Brefeldin A / pharmacology
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Cell Adhesion Molecules / drug effects
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Cell Adhesion Molecules / metabolism
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Cell Membrane / metabolism
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Chromones / pharmacology
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Culture Media, Serum-Free
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Enzyme Inhibitors / pharmacology
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Epidermal Growth Factor / pharmacology
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Green Fluorescent Proteins
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Guanine Nucleotide Exchange Factors
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Guanosine Diphosphate / metabolism
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HeLa Cells / drug effects
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HeLa Cells / metabolism
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Humans
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Luminescent Proteins / genetics
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Luminescent Proteins / metabolism
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Molecular Sequence Data
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Morpholines / pharmacology
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Mutation
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Phosphatidylinositol 3-Kinases / drug effects
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Phosphatidylinositol 3-Kinases / metabolism*
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Phosphatidylinositol Phosphates / antagonists & inhibitors
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Phosphatidylinositol Phosphates / genetics
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Phosphatidylinositol Phosphates / metabolism
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Signal Transduction
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Subcellular Fractions
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Wortmannin
Substances
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ADP-Ribosylation Factor 6
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Actins
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Androstadienes
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Cell Adhesion Molecules
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Chromones
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Culture Media, Serum-Free
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Enzyme Inhibitors
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Guanine Nucleotide Exchange Factors
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Luminescent Proteins
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Morpholines
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Phosphatidylinositol Phosphates
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Recombinant Proteins
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cytohesin-1
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phosphatidylinositol 3,4,5-triphosphate
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Guanosine Diphosphate
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Green Fluorescent Proteins
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Brefeldin A
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2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
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Epidermal Growth Factor
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ADP-Ribosylation Factors
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ARF6 protein, human
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Wortmannin