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Mol Cell. 1999 Dec;4(6):1017-27.

Maskin is a CPEB-associated factor that transiently interacts with elF-4E.

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  • 1Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester 01655, USA.

Erratum in

  • Mol Cell 2000 Apr;5(4):following 766.

Abstract

In Xenopus, the CPE is a bifunctional 3' UTR sequence that maintains maternal mRNA in a dormant state in oocytes and activates polyadenylation-induced translation during oocyte maturation. Here, we report that CPEB, which binds the CPE and stimulates polyadenylation, interacts with a new factor we term maskin. Maskin contains a peptide sequence that is conserved among elF-4E-binding proteins. Affinity chromatography demonstrates that CPEB, maskin, and elF-4E reside in a complex in oocytes, and yeast two-hybrid analyses indicate that CPEB and maskin bind directly, as do maskin and elF-4E. While CPEB and maskin remain together during oocyte maturation, the maskin-elF-4E interaction is substantially reduced. The dissolution of this complex may result in the binding of elF-4E to elF-4G and the translational activation of CPE-containing mRNAs.

PMID:
10635326
[PubMed - indexed for MEDLINE]
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