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1: Biochem Biophys Res Commun. 2000 Jan 7;267(1):156-63.Click here to read Links

Cloning of the beta(2a) subunit of the voltage-dependent calcium channel from human heart: cooperative effect of alpha(2)/delta and beta(2a) on the membrane expression of the alpha(1C) subunit.

Yamaguchi H, Okuda M, Mikala G, Fukasawa K, Varadi G.

Institute of Molecular Pharmacology, Department of Cell Biology, University of Cincinnati, College of Medicine, 231 Bethesda Avenue, Cincinnati, Ohio, 45267-0828, USA.

Expression and membrane localization of an epitope-tagged human Ca(2+) channel alpha(1C) subunit were monitored in Xenopus oocytes by confocal microscopy and electrophysiological recording. When alpha(2)/delta and beta(2a) were separately coexpressed with the alpha(1C) subunit, assessment by confocal microscopy showed an 86 and 225% increase of the channel density, respectively. Simultaneous coexpression of alpha(2)/delta and beta(2a) subunits resulted in a cooperative (470%) increase. Electrophysiological measurements performed in parallel revealed that the current augmentation by the alpha(2)/delta subunit is totally attributable to an increase in channel density, whereas the beta(2a) subunit, in addition to increasing channel density, also facilitates channel opening. Copyright 2000 Academic Press.

PMID: 10623591 [PubMed - indexed for MEDLINE]