Neuroreport. 1999 Nov 26;10(17):3659-64.

Ca2+ and pH modulate alternative splicing of exon 5 in NMDA receptor subunit 1.

Vallano ML, Beaman-Hall CM, Benmansour S.

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Department of Pharmacology, SUNY/Health Science Center, Syracuse, NY 13210, USA.

Abstract

RT-PCR and intracellular Ca2+ measurements were used to identify factors that modulate alternative splicing of exon 5 in the NMDA receptor transcript encoding NR1, in cultured cerebellar granule neurons. Although cells grown in media containing 5 mM KCl demonstrate compromised survival, they show the predicted developmental transition from NR1a (-exon 5) to NR1b (+exon 5) mRNA expression. This transition was blocked under culture conditions that promote survival; inclusion or exclusion of exon 5 is a reversible process that is sensitive to alterations in Ca2+ and pH. We conclude that alternative splicing of NR1 pre-mRNA transcripts may be regulated by developmental cues that modulate the degree of glutamate receptor activation.

PMID:: 10619662; [PubMed - indexed for MEDLINE]

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