Your browser version may not work well with NCBI's Web applications. More information here...
1: Bioorg Med Chem Lett. 1999 Dec 6;9(23):3341-6.Click here to read Links

Probing the radical mechanism of galactose oxidase using an ultrafast radical probe.

Turner BE, Branchaud BP.

Department of Chemistry, University of Oregon, Eugene 97403-1253, USA.

Processing of trans-2-phenylcyclopropylmethanols 5 and 6 by the monocopper/tyrosine radical enzyme galactose oxidase led to mechanism-based inactivation with a partition ratio, (k(cat) + k(inact))/k(inact), of approximately 1 and a primary deuterium isotope effect, k(inact(H))/k(inact(D)), of 3.2. The data are consistent with a radical mechanism for galactose oxidase with a short lived ketyl radical anion intermediate.

PMID: 10612596 [PubMed - indexed for MEDLINE]