J Neuroendocrinol. 1999 Dec;11(12):935-9.

Transgenic expression of green fluorescent protein in mouse oxytocin neurones.

Young WS 3rd, Iacangelo A, Luo XZ, King C, Duncan K, Ginns EI.

Source

Section on Neural Gene Expression, National Institute of Mental Health, Bethesda, MD 20892-4068, USA. scott@codon.nih.gov

Abstract

Routine targeting of neurones for expression of exogenous genes would facilitate our ability to manipulate their internal milieu or functions, providing insight into physiology of neurones. The magnocellular neurones of the paraventricular and supraoptic nuclei of the hypothalamus have been the objects of limited success by this approach. Here we report on the placement of the enhanced green fluorescent protein (eGFP) coding sequence at various locations within an oxytocin transgene. Placement within the first exon yielded little to no expression, whereas placement in the third exon (as an in-frame fusion with the carboxyl terminus of the oxytocin preprohormone) resulted in cell-specific expression of eGFP in oxytocin neurones. Furthermore, placement of the eGFP sequence downstream of a picornavirus internal ribosomal entry site (IRES), also in the third exon, allowed expression of the eGFP as a separate protein. Other coding sequences should now be amenable to expression within oxytocin neurones to study their physiology.

PMID:: 10583728; [PubMed - indexed for MEDLINE]

LinkOut - more resources

Full Text Sources

Other Literature Sources

COS Scholar Universe

Molecular Biology Databases

Research Materials

B6;SJL-Tg(Oxt/EGFP)AI03Wsy/J - Jackson Laboratory JAX®Mice Database

Miscellaneous

Mouse Genome Informatics (MGI)

Supplemental Content

Save items

Related citations in PubMed

Cell-specific expression and subcellular localization of neurophysin-CAT-fusion proteins expressed from oxytocin and vasopressin gene promoter-driven constructs in transgenic mice. [Exp Neurol. 2001]
Cell-specific expression and subcellular localization of neurophysin-CAT-fusion proteins expressed from oxytocin and vasopressin gene promoter-driven constructs in transgenic mice.
Jeong SW, Castel M, Zhang BJ, Fields RL, Paras P, Arnheiter H, Chin H, Gainer H. Exp Neurol. 2001 Oct; 171(2):255-71.
Vasopressin and oxytocin neurones of hypothalamic supraoptic and paraventricular nuclei co-express mRNA for Type-1 and Type-2 corticotropin-releasing hormone receptors. [J Neuroendocrinol. 2000]
Vasopressin and oxytocin neurones of hypothalamic supraoptic and paraventricular nuclei co-express mRNA for Type-1 and Type-2 corticotropin-releasing hormone receptors.
Arima H, Aguilera G. J Neuroendocrinol. 2000 Sep; 12(9):833-42.
Bovine oxytocin transgenes in mice. Hypothalamic expression, physiological regulation, and interactions with the vasopressin gene. [J Biol Chem. 1995]
Bovine oxytocin transgenes in mice. Hypothalamic expression, physiological regulation, and interactions with the vasopressin gene.
Ho MY, Carter DA, Ang HL, Murphy D. J Biol Chem. 1995 Nov 10; 270(45):27199-205.
Review Cell-specific gene expression in oxytocin and vasopressin magnocellular neurons. [Adv Exp Med Biol. 1998]
Review Cell-specific gene expression in oxytocin and vasopressin magnocellular neurons.
Gainer H. Adv Exp Med Biol. 1998; 449:15-27.
Review Estrogen modulates oxytocin gene expression in regions of the rat supraoptic and paraventricular nuclei that contain estrogen receptor-beta. [Prog Brain Res. 2002]
Review Estrogen modulates oxytocin gene expression in regions of the rat supraoptic and paraventricular nuclei that contain estrogen receptor-beta.
Shughrue PJ, Dellovade TL, Merchenthaler I. Prog Brain Res. 2002; 139:15-29.

See reviews... See all...

Cited by 6 PubMed Central articles

Review REVIEW: Oxytocin: Crossing the bridge between basic science and pharmacotherapy. [CNS Neurosci Ther. 2010]
Review REVIEW: Oxytocin: Crossing the bridge between basic science and pharmacotherapy.
Viero C, Shibuya I, Kitamura N, Verkhratsky A, Fujihara H, Katoh A, Ueta Y, Zingg HH, Chvatal A, Sykova E, et al. CNS Neurosci Ther. 2010 Oct; 16(5):e138-56. Epub 2010 Jul 7.
Specific expression of an oxytocin-enhanced cyan fluorescent protein fusion transgene in the rat hypothalamus and posterior pituitary. [J Endocrinol. 2010]
Specific expression of an oxytocin-enhanced cyan fluorescent protein fusion transgene in the rat hypothalamus and posterior pituitary.
Katoh A, Fujihara H, Ohbuchi T, Onaka T, Young WS 3rd, Dayanithi G, Yamasaki Y, Kawata M, Suzuki H, Otsubo H, et al. J Endocrinol. 2010 Mar; 204(3):275-85. Epub 2009 Dec 21.
Impaired episodic LH secretion in female mice with GFP in GnRH neurons. [Am J Physiol Endocrinol Metab....]
Impaired episodic LH secretion in female mice with GFP in GnRH neurons.
Suter KJ, O'Farrell L. Am J Physiol Endocrinol Metab. 2008 Jul; 295(1):E130-6. Epub 2008 May 27.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Transgenic expression of green fluorescent protein in mouse oxytocin neurones.
Transgenic expression of green fluorescent protein in mouse oxytocin neurones.
J Neuroendocrinol. 1999 Dec ;11(12):935-9.

PubMed
Vitamin E supplementation in hyperlipidaemic patients: effect of increasing dose...
Vitamin E supplementation in hyperlipidaemic patients: effect of increasing doses on in vitro and in vivo low-density lipoprotein oxidation.
Eur J Clin Invest. 1999 Dec ;29(12):1027-34.

PubMed
Impact of amino acids 22-27 of Rho-subfamily GTPases on glucosylation by the lar...
Impact of amino acids 22-27 of Rho-subfamily GTPases on glucosylation by the large clostridial cytotoxins TcsL-1522, TcdB-1470 and TcdB-8864.
Eur J Biochem. 1999 Dec ;266(3):1073-80.

PubMed
A case of diaphragm hernia containing accessory spleen and great omentum detecte...
A case of diaphragm hernia containing accessory spleen and great omentum detected by Tc-99m phytate scintigraphy.
Ann Nucl Med. 1999 Oct ;13(5):347-9.

PubMed
Detection and clinical characterization of patients with oral allergy syndrome c...
Detection and clinical characterization of patients with oral allergy syndrome caused by stable allergens in Rosaceae and nuts.
Ann Allergy Asthma Immunol. 1999 Nov ;83(5):377-83.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Transgenic expression of green fluorescent protein in mouse oxytocin neurones.

Source

Abstract

MeSH Terms, Substances

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

Molecular Biology Databases

Research Materials

Miscellaneous

Supplemental Content

Save items

Related citations in PubMed

Cited by 6 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information