Biochem Biophys Res Commun. 1999 Dec 9;266(1):216-21.

Identification of EPS8 as a Dvl1-associated molecule.

Inobe M, Katsube K, Miyagoe Y, Nabeshima Y, Takeda S.

Source

Department of Molecular Genetics, National Institute of Neuroscience, Kodaira, Tokyo, 187-8502, Japan.

Abstract

Dishevelled (Dsh) is involved in both Wingless (Wg) and Frizzled (Fz) signaling pathways. To further determine the function of Dsh, we have performed yeast two-hybrid screening and isolated several genes encoding the molecules associated with the PDZ domain of Dvl1, one of the murine Dsh homologs. During the screening, we found that EPS8, which is a substrate for activated EGF receptor (EGFR), specifically interacted with Dvl1. This interaction was also confirmed in vitro. Through transfection studies, we observed the mutual action between Dvl1 and EPS8. Dvl1 was hyperphosphorylated in the presence of EPS8, whereas the tyrosine phosphorylation of EPS8 by activated EGFR was inhibited in the presence of Dvl1. Immunohistochemistry showed that Dvl1 and EPS8 expression overlap in particular tissues during organogenesis. These results indicate that interaction between Dvl1 and receptor tyrosine kinase signal plays certain roles in developmental events.

PMID:: 10581192; [PubMed - indexed for MEDLINE]

MeSH Terms, Substances

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Supplemental Content

Save items

Related citations in PubMed

Constitutive phosphorylation of eps8 in tumor cell lines: relevance to malignant transformation. [Mol Cell Biol. 1995]
Constitutive phosphorylation of eps8 in tumor cell lines: relevance to malignant transformation.
Matoskova B, Wong WT, Salcini AE, Pelicci PG, Di Fiore PP. Mol Cell Biol. 1995 Jul; 15(7):3805-12.
Growth hormone-induced phosphorylation of epidermal growth factor (EGF) receptor in 3T3-F442A cells. Modulation of EGF-induced trafficking and signaling. [J Biol Chem. 2003]
Growth hormone-induced phosphorylation of epidermal growth factor (EGF) receptor in 3T3-F442A cells. Modulation of EGF-induced trafficking and signaling.
Huang Y, Kim SO, Jiang J, Frank SJ. J Biol Chem. 2003 May 23; 278(21):18902-13. Epub 2003 Mar 14.
Eps8, a substrate for the epidermal growth factor receptor kinase, enhances EGF-dependent mitogenic signals. [EMBO J. 1993]
Eps8, a substrate for the epidermal growth factor receptor kinase, enhances EGF-dependent mitogenic signals.
Fazioli F, Minichiello L, Matoska V, Castagnino P, Miki T, Wong WT, Di Fiore PP. EMBO J. 1993 Oct; 12(10):3799-808.
Review Eps8 in the midst of GTPases. [Int J Biochem Cell Biol. 2002]
Review Eps8 in the midst of GTPases.
Di Fiore PP, Scita G. Int J Biochem Cell Biol. 2002 Oct; 34(10):1178-83.
Review Bone morphogenetic proteins. [Growth Factors. 2004]
Review Bone morphogenetic proteins.
Chen D, Zhao M, Mundy GR. Growth Factors. 2004 Dec; 22(4):233-41.

See reviews... See all...

Cited by 4 PubMed Central articles

c-Jun N-terminal kinase 2 (JNK2) enhances cell migration through epidermal growth factor substrate 8 (EPS8). [J Biol Chem. 2011]
c-Jun N-terminal kinase 2 (JNK2) enhances cell migration through epidermal growth factor substrate 8 (EPS8).
Mitra S, Lee JS, Cantrell M, Van den Berg CL. J Biol Chem. 2011 Apr 29; 286(17):15287-97. Epub 2011 Feb 28.
EPS8 upregulates FOXM1 expression, enhancing cell growth and motility. [Carcinogenesis. 2010]
EPS8 upregulates FOXM1 expression, enhancing cell growth and motility.
Wang H, Teh MT, Ji Y, Patel V, Firouzabadian S, Patel AA, Gutkind JS, Yeudall WA. Carcinogenesis. 2010 Jun; 31(6):1132-41. Epub 2010 Mar 29.
Regulation of actin cytoskeleton architecture by Eps8 and Abi1. [BMC Cell Biol. 2005]
Regulation of actin cytoskeleton architecture by Eps8 and Abi1.
Roffers-Agarwal J, Xanthos JB, Miller JR. BMC Cell Biol. 2005 Oct 14; 6:36. Epub 2005 Oct 14.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Gene
Gene records that cite the current articles. Citations in Gene are added manually by NCBI or imported from outside public resources.
Gene (GeneRIF)
Gene records that have the current articles as Reference into Function citations (GeneRIFs). NLM staff reviewing the literature while indexing MEDLINE add GeneRIFs manually.
HomoloGene
HomoloGene clusters of homologous genes and sequences that cite the current articles. These are references on the Gene and sequence records in the HomoloGene entry.
Nucleotide (RefSeq)
NCBI nucleotide Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Nucleotide (Weighted)
Nucleotide records associated with the current articles through the Gene database. These are the related sequences on the Gene record that are added manually by NCBI.
OMIM (calculated)
Online Mendelian Inheritance in Man (OMIM) records that include the current articles as references in the light bulb links within or in the citations at the end of the OMIM record. The references available through the light bulb link are collected using the PubMed related articles algorithm to identify records with similar terminology to the OMIM record.
Protein (RefSeq)
NCBI protein Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Protein (Weighted)
Protein records associated with the current articles through related Gene database records. These are the related sequences on the Gene record that are added manually by NCBI.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
UniGene
UniGene clusters of expressed sequences that are associated with the current articles through references on the clustered sequence records and related Gene records.
GEO Profiles
Gene Expression Omnibus (GEO) Profiles of molecular abundance data. The current articles are references on the Gene record associated with the GEO profile.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Identification of EPS8 as a Dvl1-associated molecule.
Identification of EPS8 as a Dvl1-associated molecule.
Biochem Biophys Res Commun. 1999 Dec 9 ;266(1):216-21.

PubMed
Structural insights into phosphoinositide 3-kinase catalysis and signalling.
Structural insights into phosphoinositide 3-kinase catalysis and signalling.
Nature. 1999 Nov 18 ;402(6759):313-20.

PubMed
Activated T cells regulate bone loss and joint destruction in adjuvant arthritis...
Activated T cells regulate bone loss and joint destruction in adjuvant arthritis through osteoprotegerin ligand.
Nature. 1999 Nov 18 ;402(6759):304-9.

PubMed
Structure of the phylogenetically most conserved domain of SRP RNA.
Structure of the phylogenetically most conserved domain of SRP RNA.
RNA. 1999 Nov ;5(11):1419-29.

PubMed
Adverse upper gastrointestinal effects of rofecoxib compared with NSAIDs.
Adverse upper gastrointestinal effects of rofecoxib compared with NSAIDs.
JAMA. 1999 Nov 24 ;282(20):1929-33.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: