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Plant Physiol Biochem. 1999 Nov;37(11):831-840.

The TAG1 locus of Arabidopsis encodes for a diacylglycerol acyltransferase.

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  • 1Laboratoire de biologie des semences, INRA-INAPG, route de Saint-Cyr, 78026 Versailles cedex, France.

Abstract

Diacylglycerol acyltransferase (DGAT, EC 2.3.1.20) is a membrane enzyme that drives the final step in the formation of oils using diacylglycerol (DAG) and acyl-CoA to yield triacylglycerol (TAG). We identified a putative plant DGAT gene (TRIACYLGLYCEROL1: TAG1) and demonstrated its function by the cloning of two mutated alleles, designated AS11 (tag1-1) and ABX45 (tag1-2). One allele, AS11, has been previously characterised at the biochemical level. Mutant seeds contained less oil with a modified fatty acid profile and have reduced germination rates compared to wild-type controls. The TAG1 cDNA encodes for a 520-aa protein that possesses multiple putative transmembrane domains and shows 70 % similarity to a human DGAT cDNA.

PMID:
10580283
[PubMed - as supplied by publisher]
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