Abstract
Self-incompatibility, the rejection of self pollen, is the most widespread mechanism by which flowering plants prevent inbreeding. In Brassica, the S receptor kinase (SRK) has been implicated in the self-incompatibility response, but the molecular mechanisms involving SRK are unknown. One putative downstream effector for SRK is ARC1, a protein that binds to the SRK kinase domain. Here it is shown that suppression of ARC1 messenger RNA levels in the self-incompatible Brassica napus W1 line is correlated with a partial breakdown of self-incompatibility, resulting in seed production. This provides strong evidence that ARC1 is a positive effector of the Brassica self-incompatibility response.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Brassica / genetics
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Brassica / physiology*
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Carrier Proteins / genetics*
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Carrier Proteins / physiology*
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DNA, Antisense
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Germination
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Glycoproteins / genetics
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Glycoproteins / metabolism
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Microscopy, Fluorescence
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Phenotype
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Plant Proteins / genetics*
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Plant Proteins / metabolism
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Plant Proteins / physiology*
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Plant Structures / genetics
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Plant Structures / physiology
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Plants, Genetically Modified
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Pollen / genetics
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Pollen / physiology*
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Protein Kinases / genetics
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Protein Kinases / metabolism
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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RNA, Plant / genetics
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RNA, Plant / metabolism
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Seeds / physiology
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Transgenes
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Ubiquitin-Protein Ligases*
Substances
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Carrier Proteins
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DNA, Antisense
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Glycoproteins
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Plant Proteins
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RNA, Messenger
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RNA, Plant
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self-incompatibility locus specific glycoprotein, Brassica
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Ubiquitin-Protein Ligases
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Protein Kinases
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S-receptor kinase