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Biotechniques. 1999 Nov;27(5):1008-14.

Single-molecule fluorescence observed with mercury lamp illumination.

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  • 1California Institute of Technology, Pasadena, USA.

Abstract

We demonstrate that it is possible to observe single fluorescent molecules using a standard fluorescence microscope with mercury lamp excitation and an inexpensive cooled charge-coupled device (CCD) camera. With this equipment, we have been able to observe single molecules of tetramethyl-rhodamine, rhodamine 6G, fluorescein isothiocyanate and green fluorescent protein. Immobilized molecules were observed both in air and in aqueous solution.

PMID:
10572649
[PubMed - indexed for MEDLINE]
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