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FEBS Lett. 1999 Oct 22;460(1):41-5.

Identification of proteins that interact with NF-YA.

Author information

  • 1Department of Biochemistry, Fukui Medical University, Japan. kazuya@fmsrsa.fukui-med.ac.jp

Abstract

We used the yeast two-hybrid system to show that the serum response factor (SRF) and zinc-fingers and homeobox 1 (ZHXI) proteins interact with the A subunit of nuclear factor-Y (NF-YA). GST pulldown assays revealed that both proteins interact specifically with NF-YA in vitro. Amino acids located between 272 and 564, a region that contains two homeodomains, are required for the interaction of ZHX1 with NF-YA. Two different domains of NF-YA, a glutamine-rich region and a serine/threonine-rich region, are necessary for the interactions with ZHX1 and SRF, respectively.

PMID:
10571058
[PubMed - indexed for MEDLINE]
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