Send to:

Choose Destination
See comment in PubMed Commons below
Methods Enzymol. 2000;314:342-62.

Antisense methods for discrimination of phenotypic properties of closely related gene products: Jun kinase family.

Author information

  • 1Sidney Kimmel Cancer Center, San Diego, California 92121, USA.


Methods for the selection and characterization of antisense oligonucleotides for specifically eliminating closely related gene family members are available. High-throughput semiautomated methods using 96-well plate formats and array technology and improved assays are under active development that will streamline many steps and will likely merge. Second-generation 20-mer antisense phosphorothioate oligonucleotides containing 2'-methoxyethyl groups at the first and last 6 nucleotides with improved nuclease resistance and RNA affinity are becoming available.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk