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Nat Cell Biol. 1999 Oct;1(6):376-82.

Rab5 regulates motility of early endosomes on microtubules.

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  • 1Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse, Dresden D-01307, Germany.

Abstract

The small GTPase Rab5 regulates membrane docking and fusion in the early endocytic pathway. Here we reveal a new role for Rab5 in the regulation of endosome interactions with the microtubule network. Using Rab5 fused to green fluorescent protein we show that Rab5-positive endosomes move on microtubules in vivo. In vitro, Rab5 stimulates both association of early endosomes with microtubules and early-endosome motility towards the minus ends of microtubules. Moreover, similarly to endosome membrane docking and fusion, Rab5-dependent endosome movement depends on the phosphatidylinositol-3-OH kinase hVPS34. Thus, Rab5 functionally links regulation of membrane transport, motility and intracellular distribution of early endosomes.

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PMID:
10559966
[PubMed - indexed for MEDLINE]
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