Mol Endocrinol. 1999 Nov;13(11):1924-33.

Steroid receptor coactivator-1 and its family members differentially regulate transactivation by the tumor suppressor protein p53.

Source

Department of Biology, Chonnam National University, Kwangju, Korea.

Abstract

The tumor suppressor protein p53 exerts its cell cycle-regulatory effects through its ability to function as a sequence-specific DNA-binding transcription factor. Herein, we show that p53 physically interacts with specific subregions of steroid receptor coactivator-1 (SRC-1) and its family members, p/CIP (p300/CBP interacting protein), xSRC-3, and AIB1 (amplified in breast cancer), originally isolated as transcription coactivators of nuclear receptors, as demonstrated by the yeast and mammalian two-hybrid tests as well as glutathione S-transferase pull-down assays. Interestingly, cotransfection of HeLa cells with SRC-1- or p/CIP expression vector potentiated the p53-mediated transactivation, whereas AIB1 and xSRC-3 were repressive. All of these SRC-1 members, however, similarly stimulated transactivation mediated by nuclear receptors and AP-1, as previously described. These results suggest that SRC-1 and its family members may differentially modulate the p53 transactivation in vivo.

PMID:: 10551785; [PubMed - indexed for MEDLINE]

Free full text

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Supplemental Content

Related citations

Molecular cloning of xSRC-3, a novel transcription coactivator from Xenopus, that is related to AIB1, p/CIP, and TIF2. [Mol Endocrinol. 1998]
Molecular cloning of xSRC-3, a novel transcription coactivator from Xenopus, that is related to AIB1, p/CIP, and TIF2.
Kim HJ, Lee SK, Na SY, Choi HS, Lee JW. Mol Endocrinol. 1998 Jul; 12(7):1038-47.
Isoform-selective interactions between estrogen receptors and steroid receptor coactivators promoted by estradiol and ErbB-2 signaling in living cells. [Mol Endocrinol. 2003]
Isoform-selective interactions between estrogen receptors and steroid receptor coactivators promoted by estradiol and ErbB-2 signaling in living cells.
Bai Y, Giguére V. Mol Endocrinol. 2003 Apr; 17(4):589-99. Epub 2003 Jan 16.
Activating protein-1, nuclear factor-kappaB, and serum response factor as novel target molecules of the cancer-amplified transcription coactivator ASC-2. [Mol Endocrinol. 2000]
Activating protein-1, nuclear factor-kappaB, and serum response factor as novel target molecules of the cancer-amplified transcription coactivator ASC-2.
Lee SK, Na SY, Jung SY, Choi JE, Jhun BH, Cheong J, Meltzer PS, Lee YC, Lee JW. Mol Endocrinol. 2000 Jun; 14(6):915-25.
Review Review of the in vivo functions of the p160 steroid receptor coactivator family. [Mol Endocrinol. 2003]
Review Review of the in vivo functions of the p160 steroid receptor coactivator family.
Xu J, Li Q. Mol Endocrinol. 2003 Sep; 17(9):1681-92. Epub 2003 Jun 12.
Review Molecular structure and biological function of the cancer-amplified nuclear receptor coactivator SRC-3/AIB1. [J Steroid Biochem Mol Biol. 2002]
Review Molecular structure and biological function of the cancer-amplified nuclear receptor coactivator SRC-3/AIB1.
Liao L, Kuang SQ, Yuan Y, Gonzalez SM, O'Malley BW, Xu J. J Steroid Biochem Mol Biol. 2002 Dec; 83(1-5):3-14.

See reviews... See all...

Cited by 8 PubMed Central articles

Review Normal and cancer-related functions of the p160 steroid receptor co-activator (SRC) family. [Nat Rev Cancer. 2009]
Review Normal and cancer-related functions of the p160 steroid receptor co-activator (SRC) family.
Xu J, Wu RC, O'Malley BW. Nat Rev Cancer. 2009 Sep; 9(9):615-30.
A tumor suppressive coactivator complex of p53 containing ASC-2 and histone H3-lysine-4 methyltransferase MLL3 or its paralogue MLL4. [Proc Natl Acad Sci U S A. 2009]
A tumor suppressive coactivator complex of p53 containing ASC-2 and histone H3-lysine-4 methyltransferase MLL3 or its paralogue MLL4.
Lee J, Kim DH, Lee S, Yang QH, Lee DK, Lee SK, Roeder RG, Lee JW. Proc Natl Acad Sci U S A. 2009 May 26; 106(21):8513-8. Epub 2009 May 11.
Steroid receptor coactivator-1 is necessary for regulation of corticotropin-releasing hormone by chronic stress and glucocorticoids. [Proc Natl Acad Sci U S A. 2009]
Steroid receptor coactivator-1 is necessary for regulation of corticotropin-releasing hormone by chronic stress and glucocorticoids.
Lachize S, Apostolakis EM, van der Laan S, Tijssen AM, Xu J, de Kloet ER, Meijer OC. Proc Natl Acad Sci U S A. 2009 May 12; 106(19):8038-42. Epub 2009 Apr 29.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Steroid receptor coactivator-1 and its family members differentially regulate tr...
Steroid receptor coactivator-1 and its family members differentially regulate transactivation by the tumor suppressor protein p53.
Mol Endocrinol. 1999 Nov ;13(11):1924-33.

PubMed
ACTR/AIB1 functions as an E2F1 coactivator to promote breast cancer cell prolife...
ACTR/AIB1 functions as an E2F1 coactivator to promote breast cancer cell proliferation and antiestrogen resistance.
Mol Cell Biol. 2004 Jun ;24(12):5157-71.

PubMed
High tumor incidence and activation of the PI3K/AKT pathway in transgenic mice d...
High tumor incidence and activation of the PI3K/AKT pathway in transgenic mice define AIB1 as an oncogene.
Cancer Cell. 2004 Sep ;6(3):263-74.

PubMed
Phosphorylation of steroid receptor coactivator-1. Identification of the phospho...
Phosphorylation of steroid receptor coactivator-1. Identification of the phosphorylation sites and phosphorylation through the mitogen-activated protein kinase pathway.
J Biol Chem. 2000 Feb 11 ;275(6):4475-83.

PubMed
SRC-3 is required for prostate cancer cell proliferation and survival.
SRC-3 is required for prostate cancer cell proliferation and survival.
Cancer Res. 2005 Sep 1 ;65(17):7976-83.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Display Settings:

Send to: