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Proc Natl Acad Sci U S A. 1975 Feb;72(2):742-5.

5'-Terminal m-7G(5')ppp(5')G-m-p in vivo: identification in reovirus genome RNA.

Abstract

Methylated reovirus mRNA was synthesized in vitro in the presence of S-adenosyl-L-[methyl-3H]-methionine. Viral genome double-stranded RNA that was uniformly labeled with 32-P was isolated from purified virions. The RNAs were mixed and their 5'-terminal structures compared by electrophoretic and chromatographic analyses after enzymatic digestion. Both the mRNA and the corresponding strand in the genome RNA contain m-7G(5')ppp(5')G-m-pCp, indicating that infected cells synthesize viral RNA with blocked, methylated 5' termini.

PMID:
1054852
[PubMed - indexed for MEDLINE]
PMCID:
PMC432392
Free PMC Article
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