Evidence from studies with N-ethyl-maleimide and 12-O-tetradecanoylphorbol-13-acetate that AP-1 and CREB are involved in the glucocorticoid activation of TRH gene expression in hypothalamic cultures

L G Luo; S Wang; E Su; I M Jackson

doi:10.1016/s0006-8993(99)01789-8

Evidence from studies with N-ethyl-maleimide and 12-O-tetradecanoylphorbol-13-acetate that AP-1 and CREB are involved in the glucocorticoid activation of TRH gene expression in hypothalamic cultures

Brain Res. 1999 Sep 11;841(1-2):189-92. doi: 10.1016/s0006-8993(99)01789-8.

Authors

L G Luo¹, S Wang, E Su, I M Jackson

Affiliation

¹ Division of Endocrinology, Brown University School of Medicine, Rhode Island Hospital, Providence 02903, USA.

PMID: 10547001
DOI: 10.1016/s0006-8993(99)01789-8

Abstract

To determine whether c-fos/c-jun (AP-1) and CREB mediate glucocorticoid stimulated TRH gene regulation, we investigated the effect of N-ethyl-maleimide (NEM), an alkylating agent, and 12-O-tetradecanoylphorbol-13-acetate (TPA) on this process. NEM decreased while TPA increased TRH levels in rat hypothalamic culture, changes similar to their effects on CREB and Fos/Jun proteins in the AtT20 cell line. This suggests that glucocorticoid stimulation of TRH gene expression may be regulated by the AP-1 complex and CREB pathway.

MeSH terms

Animals
Cells, Cultured
Cyclic AMP Response Element-Binding Protein / metabolism*
Dexamethasone / pharmacology*
Dimerization
Ethylmaleimide / pharmacology*
Fetus
Gene Expression Regulation / drug effects
Gene Expression Regulation / physiology*
Genes, fos
Genes, jun
Hypothalamus / cytology
Hypothalamus / metabolism*
Neurons / cytology
Neurons / drug effects
Neurons / metabolism*
Rats
Tetradecanoylphorbol Acetate / pharmacology*
Thyrotropin-Releasing Hormone / genetics*
Transcription Factor AP-1 / metabolism*

Substances

Cyclic AMP Response Element-Binding Protein
Transcription Factor AP-1
Thyrotropin-Releasing Hormone
Dexamethasone
Tetradecanoylphorbol Acetate
Ethylmaleimide