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Nat Biotechnol. 1999 Nov;17(11):1122-4.

Increased baculovirus susceptibility of armyworm larvae feeding on transgenic rice plants expressing an entomopoxvirus gene.

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  • 1College of Bioresource Sciences, Nihon University, Kameino, Fujisawa 252-8510, Japan. tosi@brs.nihon-u.ac.jp

Abstract

We have introduced an entomopoxvirus gene encoding a virus enhancing factor (EF) into rice, which resulted in high-level accumulation of the EF in the transgenic plants. The introduced gene was stably inherited in the progeny of the primary transformants, as shown by analysis of their genomic DNA. Bioassays for insect susceptibility to baculovirus infection showed that armyworm larvae feeding on the transgenic rice had increased susceptibility to a Nucleopolyhedrovirus. Thus, introduction of the EF gene into plants can be used as a strategy to increase the effectiveness of baculoviruses in insect pest management.

PMID:
10545922
[PubMed - indexed for MEDLINE]
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