(A) Percent survival of cultures of LO28 and LO28Δ
lisK in TSB-YE (pH 3.5) after 45 min (● and ■, respectively) and 120 min (○ and □, respectively), at different points during growth (

and

, respectively). Points below the dotted line indicate that there were no survivors. (B) Growth of LO28 (○) and LO28Δ
lisK (■) in TSB-YE supplemented with 5% ethanol at 37°C. Error bars represent the standard deviations of triplicate experiments. OD
600, optical density at 600 nm.