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Proc Natl Acad Sci U S A. 1999 Oct 26;96(22):12542-7.

The importin/karyopherin Kap114 mediates the nuclear import of TATA-binding protein.

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  • 1Department of Biological Chemistry, Harvard Medical School, Boston, MA 02115, USA.


Two high copy suppressors of temperature-sensitive TATA-binding protein (TBP) mutants were isolated. One suppressor was TIF51A, which encodes eukaryotic translation initiation factor 5A. The other high copy suppressor, YGL241W, also known as KAP114, is one of 14 importin/karyopherin proteins in yeast. These proteins mediate the transport of specific macromolecules into and out of the nucleus. Cells lacking Kap114 partially mislocalize TBP to the cytoplasm. Kap114 binds TBP in vitro, and binding is disrupted in the presence of GTPgammaS. Therefore, Kap114 is an importer of TBP into the nucleus, but alternative import pathways must also exist.

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