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Mol Cell Biol. 1999 Nov;19(11):7369-76.

Saccharomyces cerevisiae RNA polymerase I terminates transcription at the Reb1 terminator in vivo.

Author information

  • 1Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA. rreeder@fred.fhcrc.org

Abstract

We have mapped transcription termination sites for RNA polymerase I in the yeast Saccharomyces cerevisiae. S1 nuclease mapping shows that the primary terminator is the Reb1p terminator located at +93 downstream of the 3' end of 25S rRNA. Reverse transcription coupled with quantitative PCR shows that approximately 90% of all transcripts terminate at this site. Transcripts which read through the +93 site quantitatively terminate at a fail-safe terminator located further downstream at +250. Inactivation of Rnt1p (an RNase III involved in processing the 3' end of 25S rRNA) greatly stabilizes transcripts extending to both sites and increases readthrough at the +93 site. In vivo assay of mutants of the Reb1p terminator shows that this site operates in vivo by the same mechanism as has previously been delineated through in vitro studies.

PMID:
10523625
[PubMed - indexed for MEDLINE]
PMCID:
PMC84730
Free PMC Article

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