Transport and storage of fresh and frozen gastric biopsy specimens for optimal recovery of Helicobacter pylori

M Heep; K Scheibl; A Degrell; N Lehn

doi:10.1128/JCM.37.11.3764-3766.1999

Transport and storage of fresh and frozen gastric biopsy specimens for optimal recovery of Helicobacter pylori

J Clin Microbiol. 1999 Nov;37(11):3764-6. doi: 10.1128/JCM.37.11.3764-3766.1999.

Authors

M Heep¹, K Scheibl, A Degrell, N Lehn

Affiliation

¹ Institute for Medical Microbiology, Uniklinik Regensburg, Regensburg, Germany. Markus.Heep@klinik.uni-regensburg.de

Abstract

The viability of Helicobacter pylori in vitro and in gastric biopsy specimens was determined. Recovery rates were 94, 87, and 77% from biopsy specimens in Portagerm pylori in cooled containers after 1, 2, and 3 days of transport, respectively (n = 307), and 97% if stored and shipped in glycerol broth at -70 degrees C (n = 232).

Publication types

Comparative Study
Multicenter Study
Research Support, Non-U.S. Gov't

MeSH terms

Bacteriological Techniques
Biopsy
Colony Count, Microbial
Evaluation Studies as Topic
Freezing
Helicobacter Infections / diagnosis
Helicobacter pylori / isolation & purification*
Humans
Stomach / microbiology*