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J Virol. 1999 Nov;73(11):9679-82.

Rescue of influenza A virus from recombinant DNA.

Author information

  • 1Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom.

Abstract

We have rescued influenza A virus by transfection of 12 plasmids into Vero cells. The eight individual negative-sense genomic viral RNAs were transcribed from plasmids containing human RNA polymerase I promoter and hepatitis delta virus ribozyme sequences. The three influenza virus polymerase proteins and the nucleoprotein were expressed from protein expression plasmids. This plasmid-based reverse genetics technique facilitates the generation of recombinant influenza viruses containing specific mutations in their genes.

PMID:
10516084
[PubMed - indexed for MEDLINE]
PMCID:
PMC113010
Free PMC Article
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