Format

Send to:

Choose Destination
See comment in PubMed Commons below
Genetics. 1999 Oct;153(2):653-64.

Appearance and properties of L-sorbose-utilizing mutants of Candida albicans obtained on a selective plate.

Author information

  • 1Department of Biochemistry, University of Rochester Medical School, Rochester, New York 14642, USA.

Abstract

This is the first report that adaptive mutagenesis can arise by chromosomal nondisjunction, a phenomenon previously associated exclusively with DNA alterations. We previously uncovered a novel regulatory mechanism in Candida albicans in which the assimilation of an alternative sugar, l-sorbose, was determined by copy number of chromosome 5, such that monosomic strains utilized l-sorbose, whereas disomic strains did not. We present evidence that this formation of monosomy of chromosome 5, which is apparently a result of nondisjunction, appeared with increased frequencies after a selective condition was applied, i.e., by adaptive mutagenesis. The rate of formation of l-sorbose-utilizing mutants per viable cell per day ranged from 10(-6) at the initial time of detection to 10(-2) after 4 days of incubation on the selective plate.

PMID:
10511546
[PubMed - indexed for MEDLINE]
PMCID:
PMC1460764
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk