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Curr Opin Struct Biol. 1999 Oct;9(5):627-34.

Extended resolution fluorescence microscopy.

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  • 1Department of Biochemistry, University of California San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143-0448, USA.


Fluorescence microscopy is an essential tool of modern biology, but, like all forms of optical imaging, it is subject to physical limits on its resolving power. In recent years, several exciting techniques have been introduced to exceed these limits, including standing wave microscopy, 4Pi confocal microscopy, I5M and structured illumination microscopy. Several such techniques have been definitively demonstrated for the first time during the past year.

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