Nat Biotechnol. 1999 Oct;17(10):1030-2.

A generic protein purification method for protein complex characterization and proteome exploration.

Rigaut G, Shevchenko A, Rutz B, Wilm M, Mann M, Séraphin B.

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European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany.

Abstract

We have developed a generic procedure to purify proteins expressed at their natural level under native conditions using a novel tandem affinity purification (TAP) tag. The TAP tag allows the rapid purification of complexes from a relatively small number of cells without prior knowledge of the complex composition, activity, or function. Combined with mass spectrometry, the TAP strategy allows for the identification of proteins interacting with a given target protein. The TAP method has been tested in yeast but should be applicable to other cells or organisms.

PMID:: 10504710; [PubMed - indexed for MEDLINE]

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Review The tandem affinity purification (TAP) method: a general procedure of protein complex purification. [Methods. 2001]
Review The tandem affinity purification (TAP) method: a general procedure of protein complex purification.
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Cited by over 100 PubMed Central articles

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