Effect of complex polyphenols and tannins from red wine (WCPT) on chemically induced oxidative DNA damage in the rat

Eur J Nutr. 1999 Aug;38(4):190-5. doi: 10.1007/s003940050061.

Abstract

Background: Flavonoids are polyphenolic antioxidants occurring in vegetables and fruits as well as beverages such as tea and wine which have been thought to influence oxidative damage.

Aim of the study: We wanted to verify whether a complex mixture of wine tannins (wine complex polyphenols and tannins, WCPT) prevent chemically-induced oxidative DNA damage in vivo.

Methods: Oxidative DNA damage was evaluated by measuring the ratio of 8-hydroxy-2'-deoxyguanosine (80HdG)/ 2-deoxyguanosine (2dG) x 10(-6) in hydrolyzed DNA using HPLC coupled with electrochemical and UV detectors.

Results: We treated rats with WCPT (57 mg/kg p.o.) for 14 d, a dose 10-fold higher than what a moderate wine drinker would be exposed to. WCPT administration significantly reduced the ratio of 80HdG/2dG x 10(-6) in liver DNA obtained from rats treated with 2-nitropropane (2NP) relative to controls administered 2NP only (33. 3 +/- 2.5 vs. 44.9 +/- 3.2 x 10(-6) 2dG; micro +/- SE; p<0.05). On the contrary, pretreatment with WCPT for 10 d did not protect the colon mucosa from oxidative DNA damage induced by 1, 2-dimethylhydrazine (DMH). 2NP and DMH are hepatic and colon carcinogens, respectively, capable of inducing oxidative DNA damage.

Conclusions: WCPT have protective action against some types of chemically-induced oxidative DNA damage in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 1,2-Dimethylhydrazine / adverse effects
  • 8-Hydroxy-2'-Deoxyguanosine
  • Animals
  • Carcinogens / adverse effects
  • Chromatography, High Pressure Liquid / veterinary
  • Colon / physiopathology
  • DNA / drug effects
  • DNA / isolation & purification
  • DNA / metabolism
  • DNA Damage / drug effects*
  • DNA Damage / physiology
  • Deoxyguanosine / analogs & derivatives
  • Deoxyguanosine / analysis
  • Flavonoids*
  • Intestinal Mucosa / physiopathology
  • Liver / physiopathology
  • Male
  • Nitroparaffins / adverse effects
  • Oxidation-Reduction / drug effects
  • Phenols / metabolism
  • Phenols / pharmacology*
  • Polymers / metabolism
  • Polymers / pharmacology*
  • Polyphenols
  • Propane / adverse effects
  • Propane / analogs & derivatives
  • Rats
  • Rats, Inbred F344
  • Tannins / metabolism
  • Tannins / pharmacology*
  • Wine*

Substances

  • Carcinogens
  • Flavonoids
  • Nitroparaffins
  • Phenols
  • Polymers
  • Polyphenols
  • Tannins
  • 8-Hydroxy-2'-Deoxyguanosine
  • DNA
  • Deoxyguanosine
  • 2-nitropropane
  • 1,2-Dimethylhydrazine
  • Propane