Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
J Magn Reson. 1999 Oct;140(2):510-2.

Observation of through-hydrogen-bond 2hJHC' in a perdeuterated protein.

Author information

  • 1Institute of Structural Biology, Forschungszentrum Jülich, Jülich, 52425, Germany.

Abstract

It is demonstrated that J connectivity between amide protons and hydrogen-bond-accepting carbonyl carbons can be observed in perdeuterated human ubiquitin. A selective pulse scheme is used to detect these small 2hJHC' interactions in the presence of the much larger through-covalent-bond 2JHC' and 3JHC' couplings. The ratio of the observed through-H-bond correlation intensity and the 2JHC' connectivity observed in a reference spectrum indicates 2hJHC' values of ca. 0.4-0.6 Hz, which are only slightly smaller than the corresponding 3hJNC' values. However, for technical reasons, 2hJHC' couplings are more difficult to measure than 3hJNC'.

Copyright 1999 Academic Press.

PMID:
10497060
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk