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Nat Biotechnol. 1999 Sep;17(9):906-9.

A galinstan expansion femtosyringe for microinjection of eukaryotic organelles and prokaryotes.

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  • 1Institute of General Botany and Plant Physiology, Justus Liebig University Giessen, Senckenbergstrasse 17, 35390 Giessen, Germany. Michael.Knoblauch@bot1.bio.uni-giessen.de

Abstract

A galinstan expansion femtosyringe enables femtoliter to attoliter samples to be introduced into prokaryotes and subcellular compartments of eukaryotes. The method uses heat-induced expansion of galinstan (a liquid metal alloy of gallium, indium, and tin) within a glass syringe to expel samples through a tip diameter of about 0.1 microm. The narrow tip inflicts less damage than conventional capillaries, and the heat-induced expansion of the galinstan allows fine control over the rate of injection. We demonstrate injection of Lucifer Yellow and Lucifer Yellow-dextran conjugates into cyanobacteria, and into nuclei and chloroplasts of higher organisms. Injection of a plasmid containing the bla gene into the cyanobacterium Phormidium laminosum resulted in transformed ampicillin-resistant cultures. Green fluorescent protein was expressed in attached leaves of tobacco and Vicia faba following injection of DNA containing its gene into individual chloroplasts.

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PMID:
10471935
[PubMed - indexed for MEDLINE]
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