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J Bacteriol. 1999 Sep;181(17):5516-20.

Molecular characterization of the PhoP-PhoQ two-component system in Escherichia coli K-12: identification of extracellular Mg2+-responsive promoters.

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  • 1Department of Agricultural Chemistry, Kinki University, 3327-204, Nakamachi, Nara 631-8505, Japan.

Abstract

We identified Mg2+-responsive promoters of the phoPQ, mgtA, and mgrB genes of Escherichia coli K-12 by S1 nuclease analysis. Expression of these genes was induced by magnesium limitation and depended on PhoP and PhoQ. The transcription start sites were also determined, which allowed us to find a (T/G)GTTTA direct repeat in their corresponding promoter regions.

PMID:
10464230
[PubMed - indexed for MEDLINE]
PMCID:
PMC94065
Free PMC Article
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