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    J Bacteriol. 1999 Sep;181(17):5509-11.

    The Escherichia coli NadR regulator is endowed with nicotinamide mononucleotide adenylyltransferase activity.

    Source

    Istituto di Biochimica, Facoltà di Medicina, Università di Ancona, 60131 Ancona, Italy.

    Abstract

    The first identification and characterization of a catalytic activity associated with NadR protein is reported. A computer-aided search for sequence similarity revealed the presence in NadR of a 29-residue region highly conserved among known nicotinamide mononucleotide adenylyltransferases. The Escherichia coli nadR gene was cloned into a T7-based vector and overexpressed. In addition to functionally specific DNA binding properties, the homogeneous recombinant protein catalyzes NAD synthesis from nicotinamide mononucleotide and ATP.

    PMID:
    10464228
    [PubMed - indexed for MEDLINE]
    PMCID:
    PMC94063
    Free PMC Article

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