Display Settings:

Format

Send to:

Choose Destination
We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Hum Genet. 1999 Jun;104(6):498-504.

Molecular cloning and characterization of the human NUDC gene.

Author information

  • 1Department of Human Genetics, The University of Chicago, IL 60637, USA.

Abstract

In both Aspergillus nidulans and the mouse, studies of the nuclear distribution gene C (NudC) have strongly suggested that the NudC protein interacts with NudF, which is the product of NudF, a homologue of human LIS1 (also know as PAFAH1B1), one of the causative genes for classical lissencephaly. We have isolated the human NUDC gene and its two processed pseudogenes. The human NUDC gene is highly conserved and its predicted amino acid sequence shows 94% identity to mouse NudC and 95% identity to rat NudC. The genomic structure of NUDC, its chromosomal localization, and expression pattern in human tissues were characterized. NUDC consists of at least 9 exons ranging from 66 bp to 266 bp in size and 8 introns from 92 bp to 2.0 kb in length, and the total genomic region spans about 8 kb. NUDC was mapped to 1p34-p35 by fluorescence in situ hybridization. Northern analysis showed a major 1.6 kb transcript in all fetal and adult tissues examined. Primers which amplify individual exons of NUDC were developed for mutation analysis.

PMID:
10453739
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for Springer
    Loading ...
    Write to the Help Desk