We observed the effects of thyrotropin (TSH), interleukin (IL)-1 beta and IL-6 on the intercellular communication of FRTL-5 cells with fluorenscence redistribution after photobleaching (FRAP) analysis. FRTL-5 cells were cultured and exposed to the different concentrations of TSH, IL-1 beta and IL-6 for 12 hours. The mean fluorescence recovery rate (MFRR, %/min) of the cells labelled with carboxyfluocein diacetate (CFDA) after photobleaching was measured with laser scanning cytometry. The MFRR (%/min) of the cells after exposure to TSH was 0.445 +/- 0.033 at the control group, 0.679 +/- 0.054 at the group of 0.1 U/L, 0.950 +/- 0.073 at the group of 1 U/L, and 0.799 +/- 0.082 at the group of 5 U/L, respectively (F = 11.44, P < 0.01). The indicated that TSH could enhance the intercellular communication of FRTL-5 cells. The MFRR after exposure to IL-1 beta was 0.564 +/- 0.032 at the control group, 0.485 +/- 0.042 at the group of 10(3) U/L, 0.445 +/- 0.043 at the group of 10(4) U/L and 0.405 +/- 0.029 at the group of 10(5) U/L, respectively (F = 3.58, P < 0.01). The suggested that IL-1 beta could inhibite the intercellular communication of FRTL-5 cells. IL-6 had no obvious effect on the intercellular communication of FRTL-5 cells.