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    J Chromatogr B Biomed Sci Appl. 1999 Jun 11;729(1-2):333-9.

    Gas chromatographic-mass spectrometric determination of total homocysteine in human plasma by stable isotope dilution: method and clinical applications.

    Ducros V, Schmitt D, Pernod G, Faure H, Polack B, Favier A.

    Source

    Laboratoire de Biochimie C, CHU de Grenoble, France.

    Abstract

    The detection and quantitation of slight increases of plasma homocysteine levels is of growing interest. This has prompted us to develop a highly sensitive and accurate capillary gas chromatography-mass spectrometry (GC-MS) method. The method proved to be highly sensitive (DL=0.17 micromol/l) with between- and within-run precision less than 6% and 7%, respectively. Reference values of plasma total homocysteine have been determined for men (n=39) and women (n=36), showing a significant difference (P=0.003) between gender. Preliminary results in cerebrovascular accidents and in venous thrombosis are presented.

    PMID:
    10410959
    [PubMed - indexed for MEDLINE]

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