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Genomics. 1999 Jul 1;59(1):18-23.

A human nucleobase transporter-like cDNA (SLC23A1): member of a transporter family conserved from bacteria to mammals.

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  • 1British Columbia Cancer Research Centre, Vancouver, British Columbia, V5Z 4L3, Canada. dhouge@bccancer.bc.ca

Abstract

A family of related polytopic membrane proteins that mediate the transport of nucleobases has been extended to Homo sapiens by the cloning of a full-length human cDNA that encodes a nucleobase transporter-like protein. The protein is predicted to contain 11-14 transmembrane-spanning regions, exhibits 20-28% overall sequence identity to fungal and bacterial transporters, and contains a conserved signature motif found in this family. Fluorescence in situ hybridization localized the gene (HGMW-approved symbol SLC23A1) to human chromosome 20p13. Human nucleobase transporter-like mRNA was present in all tissues examined, with lower levels found in heart, skeletal muscle, and ovary. Expression of the 60-kDa cDNA-encoded protein was demonstrated by an in vitro transcription-translation approach. The identification of this nucleobase transporter-like protein will allow the further elucidation of the interaction of human cells with physiological nucleobases and pharmacologically important drugs such as 5-F-uracil, dideoxynucleosides, and acyclic nucleosides.

Copyright 1999 Academic Press.

PMID:
10395795
[PubMed - indexed for MEDLINE]
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