Format

Send to:

Choose Destination
See comment in PubMed Commons below
Mech Dev. 1999 Apr;82(1-2):3-21.

Ten years of gene targeting: targeted mouse mutants, from vector design to phenotype analysis.

Author information

  • 1Max-Planck-Institute for Brain Research, Deutschordenstr. 46, D-60528, Frankfurt, Germany. umueller@mpih-frankfurt.de

Abstract

Gene targeting, defined as the introduction of site-specific modifications into the genome by homologous recombination, has revolutionarized the field of mouse genetics and allowed the analysis of diverse aspects of gene function in vivo. It is now possible to engineer specific genetic alterations ranging from subtle mutations to chromosomal rearrangements and more recently, even tissue-specific inducible gene targeting with temporo-spatial control has become feasible. This review tries to recapitulate what we have learned in this extremely rapidly expanding field during the past decade. Diverse aspects of the technique will be discussed starting from basic construct design to the analysis of complex phenotypes, including recent advances on inducible expression system. Many examples from different areas of biomedical research are given to illustrate the purpose and limitations of the employed experimental approaches.

PMID:
10354467
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk