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Nat Biotechnol. 1999 May;17(5):462-5.

Surface display of a parasite antigen in the ciliate Tetrahymena thermophila.

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  • 1Department of Cellular Biology, University of Georgia, Athens 30602, USA. jgaertig@cb.uga.edu

Abstract

The ciliated protozoan, Tetrahymena thermophila, offers an attractive medium for the expression of heterologous proteins and could prove particularly useful for the display of foreign proteins on the cell surface. Although progress has been made in transformation of Tetrahymena with heterologous DNA, methods that permit reliable expression of foreign genes have been lacking. Using a mutant strain of T. thermophila carrying a negatively selectable allele of a beta-tubulin gene, we have been able to direct foreign genes to this locus by homologous recombination. Transformed cell lines producing foreign proteins were readily identified and, in at least one case, targeting of proteins to the plasma membrane was accomplished.

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PMID:
10331805
[PubMed - indexed for MEDLINE]
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