Abstract
An enzyme-linked immunosorbent assay (ELISA) based on the recombinant Toscana virus nucleoprotein (rN) has been developed. Its sensitivity and specificity for the detection of virus-specific immunoglobulins G and M in human sera were similar to those of the ELISA that is based on an antigen extracted from infected mouse brain and that is routinely used for serodiagnosis.
MeSH terms
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Animals
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Antibodies, Viral / blood*
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Blotting, Western
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Brain / virology
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Enzyme-Linked Immunosorbent Assay / methods
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Humans
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Immunoglobulin G / blood*
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Immunoglobulin M / blood*
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Mice
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Nucleocapsid Proteins / immunology
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Phlebotomus Fever / blood
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Phlebotomus Fever / diagnosis*
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Phlebotomus Fever / immunology
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Phlebovirus / immunology*
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Phlebovirus / isolation & purification*
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Sensitivity and Specificity
Substances
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Antibodies, Viral
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Immunoglobulin G
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Immunoglobulin M
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Nucleocapsid Proteins