Detection of Toscana virus-specific immunoglobulins G and M by an enzyme-linked immunosorbent assay based on recombinant viral nucleoprotein

J Clin Microbiol. 1999 Jun;37(6):2010-2. doi: 10.1128/JCM.37.6.2010-2012.1999.

Abstract

An enzyme-linked immunosorbent assay (ELISA) based on the recombinant Toscana virus nucleoprotein (rN) has been developed. Its sensitivity and specificity for the detection of virus-specific immunoglobulins G and M in human sera were similar to those of the ELISA that is based on an antigen extracted from infected mouse brain and that is routinely used for serodiagnosis.

MeSH terms

  • Animals
  • Antibodies, Viral / blood*
  • Blotting, Western
  • Brain / virology
  • Enzyme-Linked Immunosorbent Assay / methods
  • Humans
  • Immunoglobulin G / blood*
  • Immunoglobulin M / blood*
  • Mice
  • Nucleocapsid Proteins / immunology
  • Phlebotomus Fever / blood
  • Phlebotomus Fever / diagnosis*
  • Phlebotomus Fever / immunology
  • Phlebovirus / immunology*
  • Phlebovirus / isolation & purification*
  • Sensitivity and Specificity

Substances

  • Antibodies, Viral
  • Immunoglobulin G
  • Immunoglobulin M
  • Nucleocapsid Proteins